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Determination of cell cycle phase in live melanoma cells using SR-FTIRM

The intinsic vibrational details of individual live cells have been successfully exploited to sort in situ each of them according to the progression status, so that FTIRM can be ranked as the first label-free bioanalytical tool to monitor in real time individual cellular response to external agents, exactly knowing the progression state at the time of stress application 


D.E. Bedolla et al. Analyst 138, 4015 (2013) 
 



The knowledge of cell cycle phase distribution is of paramount importance for understanding cellular behaviour under normal and stressed growth conditions. we report on the use of FTIRM as technique alternative to flow cytometry using propidium iodide staining for studying cell cycle distribution in live cells. Hierarchical Cluster Analysis (HCA) of cellular microspectra in the 1300-1000 cm-1 region pointed out a distribution of cells among clusters, which is in good agreement with FC results among G0/G1, S and G2/M phases. The differentiation is mostly driven by the intensity of PhI, that almost doubles during cellular progression. FTIRM of live cells is then   proposed as a powerful method for the in situ determination of the cell cycle stage of an individual cell, without any labelling or staining, which gives the advantage of possibly monitoring specific cellular responses to several types of stimuli by clearly separating the spectral signatures related to the cellular response from those of cells that are normally progressing

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Determination of cell cycle phases in live B16 melanoma cells using IRMS;
D.E. Bedolla et al.
Analyst 138, 4015 (2013)

 

 

Last Updated on Wednesday, 01 October 2014 10:51